EzWay Annexin V-FITC Apoptosis Detection Kit

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EzWay Annexin V-FITC Apoptosis Detection Kit

Catalog Number	K29100
Size	100T
Type	Kit
Datasheet

Features

Utilizes FITC-conjugated Annexin V protein for detection of cells undergoing apoptosis
Annexin V FITC binds to the membranes of apoptotic cells
Detection by flow cytometry or fluorescence microscopy

Reading of Result

Non-apoptotic cells: Annexin-V negative and PI negative
Non-apoptotic cells: Annexin-V negative and PI negative
Necrotic cells or late apoptotic cells: Annexin-V positive and PI positive

Kit Contents

Annexin V-FITC
5X Binding buffer
5X Binding buffer

How to Use

Cell preparation and rinsing cells with Binding Buffer
Add Annexin V-FITC
Add Propidium Iodide
Incubate at 4℃ in the dark.
Detection

Staining of healthy and apoptotic Jurkat cells using flow cytometry
Jurkat cells were incubated with 0.5ug/ml actinomycin D for 20 hours. Untreated cells are shown in the left panel, apoptotic induced cells are shown in the right panel.