Taq DNA Polymerase II (MgCl2 Free Reaction Buffer)

Taq DNA Polymerase II (MgCl2 Free Reaction Buffer)

Catalog Number	K0560310
Size	1000U
Type	PCR Kit
Datasheet

Description

Taq DNA Polymerase is the enzyme from the cloned Thermus aquaticus expressed in E.coli. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium and also possesses a 5'→3' exonuclease activity.
Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.

Features

Ultrapure, thermostable recombinant enzyme
Highly thermostable: 10min at 97°C, 60min at 94°C
5'→3' exonuclease activity
Extra A addition activity
Amplification range:
- Lambda DNA: up to 6Kb (Max. 12Kb)
- Human genomic DNA: up to 2Kb (Max. 4Kb)
Composition of Taq polymerase and Taq PCR buffer containing MgCl₂
No Nuclease, RNase, Protease contamination

Applications

Amplification of genomic DNA and cDNA targets up to 3kb long
GC-rich sequences or secondary structures
TA Cloning
Differential Display
Degenerate PCR

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTPs into an acid-insoluble product in 30 minutes at 74°C.

Source

E. coli, recombinant gene from Thermus aquaticus

Packing

Component	Cat.No	Packing
Taq DNA Polymerase II	K0560310	1000U (500U x 2)
Taq DNA Polymerase II	K0560350	5000U (500U x 10)
10x Taq PCR Buffer II	K0561012	1.5ml x 4 (for 1000U)
10x Taq PCR Buffer II	K0561012	1.5ml x 20 (for 5000U)
MgCl₂ Solution	K0561041	1.0ml x 2 (for 1000U)
MgCl₂ Solution	K0561041	1.0ml x 2 (for 1000U)

PCR for human beta-globin gene (A) and lambda DNA (B) were performed using a serial dilution of Taq DNA Polymerase.

The Magic buffer is very useful to amplify DNA of high GC contents or of complicated structure. Human retinoblastoma gene (A), human apolipoprotein E gene (B) and human Htra serine protease1 gene (C) were amplified by addition of Magic buffer to Taq DNA Polymerase.