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Sfi I
Catalog Number K1750123
Size 1000U
Type Buffer & Reagent
Datasheet Inquire
 
Recogntion Site
 
Concentration
5-15 units/ul. Assayed on Ad-2 DNA.
 
Source
Streptomyces fimbriatus
 
Reaction Condition
Buffer B + BSA
[6mM Tris-HCl (pH7.5 @ 37°C), 6mM MgCl2, 50mM NaCl, 1mM DTT]
Supplement with 100 ug/ml BSA. Incubate at 50°C.
 
Storage Condition
  • 5mM KPO4 (pH7.4)
  • 300mM NaCl
  • 0.1mM EDTA
  • 5mM 2-mercaptoethanol
  • 0.15% Triton X-100
  • 0.2mg/ml BSA
  • 50% glycerol
  • Store at -20°C
 
No. of Cleavage Sites
 
Note
Incubaion at 37℃ results in 10% activity.