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- Point Mutation Analysis
- SSCP Analysis
- Heteroduplex Analysis
SequaGel MD permits minor mutational
differences in DNA sequences to be
detected as a high resolution
relative mobility (Rf) shift.
SequaGel MD is a proprietary
formulation, supplied as a 2X stock,
designed to resolve sequence related
differences by SSCP (Single Strand
Conformational Polymorphism) and
Heteroduplex Analysis.
DNA mutations, or sequence
modifications, are readily
associated with specific disease
states. Since a variation of a
single nucleotide in a sequence may
indicate a significant genetic
anomaly, an extremely sensitive
method to analyze these mutations is
necessary.
Two principle methods of
conformational analysis have been
developed, SSCP Analysis and
Heteroduplex Analysis. SequaGel MD
is available as a monomer solution
packaged with Triple Dye Loading
Buffer in the SequaGel MD
Heteroduplex Kit or with SSCP Stop
Solution in the SequaGel MD SSCP Kit
SequaGel MD is designed to run SSCP
samples in the 100-300 nucleotide
range at a 0.5X concentration. Small
fragments may be better resolved by
using higher concentration (0.75X)
gels. Larger fragments may be better
resolved by running lower
concentration (0.4X) gels. SequaGel
MD is designed to run heteroduplex
analysis on DNA fragments up to 900
bases at a 1X concentration.
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