SensiMix SYBR No-ROX Kit


Features and Benefits Reproducible: optimized for a wide range of templates and generates real-time data reliably and reproducibly Specific: SensiTaq, a highly sensitive hot-start DNA polymerase that is inactive during setup, preventing non specific amplification including primer-dimer formation. Convenient: ideal for high throughput applications with extremely high specificity Optimized buffer system: Improves specificity and low-copy detection Instrument Compatibility Roche LightCycler® 480 platform, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Corbett Rotor-Gene™ 3000, Rotor-Gene™ 6000, Eppendorf Mastercycler® ep realplex and Techne Quantica®. (See product selection table). Description SensiMix SYBR No-ROX kit contains all the components necessary to perform consistent and reproducible real-time PCR assays with your DNA template. but it does not contain an internal reference. As it is no longer essential to read the internal reference through the red channel, the red channel becomes free and can be used to monitor a probe. This reagent is suitable for those block-based and rotor-based machines where an internal reference is not required for normalisation. Volume QT650-02: 250 x 50µl Reactions: 5 x 1.25ml QT650-05: 500 x 50µl Reactions: 10 x 1.25ml QT650-20: 2000 x 50µl Reactions: 40 x 1.25ml Components 2x Reaction mix consisting of: Heat-activated DNA polymerase Ultra-pure dNTPs MgCl₂ (6mM) SYBR® Green I Plus separate vials of: 50mM MgCl₂ Kit Size The kit size is based on a 50µl final reaction volume. Shipping Conditions 6 months at -20°C. Avoid exposure of the SYBR® Green I to light. Product Citations 1. Doe C.M. et al. Human Molecular Genetics 18(12): 2140-2148 (2009) 2. Schaerli Y. et al. Anal. Chem. 81(1): 302-306 (2009) 3. Parry L.J. et al. Reproduction, Fertility and Development 21(4): 549–560 (2009) 4. De Winter P. et al. British Poultry Science 49(5): 566 – 573 (2008)