SensiMix SYBR Kit


Features and Benefits Simple and reproducible: a choice of optimized kits for different platforms, just add primers and template, reducing possible errors in set-up Specific and sensitive: for detection of a wide range of template concentration, even from hard to obtain and low copy number samples Fast protocols: can be used with the new generation of fast cyclers without compromising performance Flexible: validated with all major real-time instruments 2x master mix: premixed with SYBR Green I and an internal reference for convenience Instrument Compatibility ABI 7000, 7300, 7700, 7900, 7900HT and StepOne™. (See product selection table) Description Versatility Mix optimised for SYBR® Green I chemistry. Suitable for high throughput applications e.g. target validation for drug discovery. Designed to work across a range of block and rotor-based instruments. SensiMix SYBR kit contains all the components necessary to perform consistent and reproducible real-time PCR assays with your DNA template. The kits deliver highly specific and sensitive results across a wide dynamic range of template concentrations (over 10 fold), producing reliable real-time data quickly and easily. The kits are suitable for DNA templates or RNA templates following reverse transcription with the Bioline cDNA Synthesis Kit (BIO-65025) and are compatible with the ABI 7000, 7700, 7900HT and StepOne™ platforms. (See product selection table) The kits are powered by SensiTaq, a highly-sensitive hot-start DNA polymerase that provides higher specificity, and ROX passive reference fluorescent dye, making it specifically suitable for use on real-time instruments that measure this passive reference signal. Volume QT605-02: 250 x 50µl Reactions: 5 x 1.25ml QT605-05: 500 x 50µl Reactions: 10 x 1.25ml QT605-20: 2000 x 50µl Reactions: 40 x 1.25ml Components 2x Reaction mix consisting of: Heat-activated DNA polymerase Ultra-pure dNTPs MgCl₂ (6mM) Internal reference SYBR® Green I Kit Size The kit size is based on a 50µl final reaction volume. Storage Conditions 6 months at -20°C. Avoid exposure of the SYBR® Green I to light. Product Citations 1. Lappas M., et al. Placenta 30(3): 256-262 (2009) 2. Rosker C., et al. J. Biol. Chem. 284(8): 5186-5194 (2009) 3. Barker H.E, et al. BMC Developmental Biology 8(1): 94 (2008) 4. Johnson C.E. et al. StemCells 25: 1913–1923 (2007) . “What was a problem before now works perfectly! I tried several SYBR Green Kits but SensiMix SYBR worked best. Triplicates have very good standard deviations and detection of even low transcript levels via real-time RT-PCR are no problem anymore in Plasmodium falciparum.” Dipl. biol. Anne Röseler, Nutritional Biochemistry, Justus-Liebig University Giessen, Germany.