SensiMix Probe Kit


Features and Benefits Unmatched sensitivity, earlier Ct detection Superior PCR Efficiency with sequence specific probes High specificity, reproducibility and reduced background Ideal for genotyping, multiplexing and gene expression analysis Compatibility with all real-time PCR instruments Instrument Compatibility ABI 7000, 7300, 7500, 7500 Fast, 7700, 7900, 7900HT, StepOneTM, StepOneTM Plus, Roche LightCycler® 480, Bio-Rad iCycler®, iQTM5 and MyiQTM, OpticonTM, OpticonTM2, Chromo4TM, MiniOpticonTM, CFX96TM, CFX384TM, Cepheid SmartCycler®, Qiagen (Corbett) Rotor-GeneTM 3000, Rotor-GeneTM6000, Eppendorf Mastercycler® ep realplex, Techne Quantica®, Agilent (Stratagene) Mx4000®, Mx3000P® and Mx3005P® (See product selection table). Description SensiMix II Probe is a second-generation 2x mastermix which has been formulated for superior sensitivity and accurate real-time PCR using sequence specific probe chemistries such as TaqMan®, Scorpion®, Assay On Demand®, allelic discrimination and molecular beacon probes. It uses the latest advances in enzyme chemistry to deliver superior performance in genotyping, gene expression analysis and multiplexing (Fig. 1). SensiMix II Probe generates highly reproducible and reliable data on all real-time PCR instruments. SensiMix II Probe uses the hot-start properties of our highly engineered proprietary DNA polymerase, SensiTaq, giving high specificity and sensitivity and greatly reducing non-specific amplification, resulting from non-specific priming events during reaction setup and initiation and increases overall reaction efficiency. This is further enhanced with the use of our new buffer formulation which has been designed to increase probe and primers specific binding, leading to reduced background, earlier cycle times (Cts) (Fig. 2) and better allelic discrimination (Fig. 3). A separate tube of ROX fluorescent dye is supplied in order to facilitate the option of measuring this passive reference signal on real-time instruments including the ABI 7500 and 7900HT and Agilent (Stratagene) Mx3000P® and Mx3005P® platforms. Volume BIO-91002: 250 x 50µl Reactions: 5 x 1.25ml BIO-91005: 500 x 50µl Reactions: 10 x 1.25ml BIO-91020: 2000 x 50µl Reactions: 40 x 1.25ml Components 2x Reaction mix consisting of: Heat-activated DNA polymerase Ultra-pure dNTPs MgCl₂ (6mM) Plus separate vials of: 50mM MgCl₂ 25mM ROX Internal reference Kit Size The kit size is based on a 50µl final reaction volume. Storage Conditions 6 months at -20°C. Avoid exposure of the ROX™ to light.