SensiMix Capillary One-Step Kit


Features and Benefits Simple: developed for both SYBR Green I and probe assays Specific: optimised buffer specifically designed for real-time instruments that use glass capillaries Reproducible:master-mix contains reverse transcriptase for efficient cDNA synthesis together with an optimized amount of highly engineered hot-start SensiTaq enzyme, to provide reproducible real-time data Sensitive: accurate linear standard curve from a 10-fold RNA dilution series over a broad dynamic range Convenient: reduced pipetting steps and possible contamination and RNA degradation Instrument Compatibility Roche LightCycler® 1.0 and 2.0(See product selection table). Description The SensiMix Capillary One-Step Kit has been developed to perform highly reproducible cDNA synthesis and subsequent real-time PCR assays in one tube, on glass capillary-based real-time instruments such as the Roche LightCycler® 1.0 and 2.0 platforms (See product selection table). The kit contains BSA to ensure compatibility with capillary based machines, along with reverse transcriptase for efficient cDNA synthesis together with an optimized amount of highly engineered hotstart SensiTaq enzyme, allowing highly reproducible first strand cDNA synthesis and subsequent real-time PCR assays in one tube. The kit has been developed to provide the high sensitivity required for challenging assays on capillary instruments both for probe and for SYBR® Green I based chemistries. In order to reduce degradation of RNA, a separate tube of RiboSafe RNase inhibitor is included to minimize enzymatic degradation of the RNA by contaminating RNase activity. Volume QT255-01: 25 x 20µl Reactions: 1 x 1.250µl QT255-02: 250 x 20µl Reactions: 2 x 1.25ml QT255-05: 500 x 20µl Reactions: 4 x 1.25ml QT255-20: 2000 x 20µl Reactions: 16 x 1.25ml Components 2x Reaction mix consisting of: Heat-activated DNA polymerase Sensi reverse transcriptase Internal reference Ultra-pure dNTPs MgCl₂ (6mM) Plus separate vials of: 50mM MgCl₂ RNase inhibitor DEPC-treated water SYBR® Green I Kit Size The kit size is based on a 20µl final reaction volume. Storage Conditions 6 months at -20°C. Avoid exposure of the SYBR® Green I to light.