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Pvu I
Catalog Number K1750105
Size 100U
Type Buffer & Reagent
Datasheet Inquire
 
Recogntion Site
 
Concentration
2-10 units/ul. Assayed on λ DNA
 
Source
Proteus vulgaris.
 
Reaction Condition
Buffer D
[6mM Tris-HCl (pH7.9 @ 37°C), 6mM MgCl2, 150mM NaCl, 1mM DTT]
Incubate at 37°C.
 
Storage Condition
  • 10mM Tris-HCl (pH7.4)
  • 50mM NaCl
  • 0.1mM EDTA
  • 1mM DTT
  • 0.5mg/ml BSA
  • 50% glycerol
  • Store at -20°C
 
Isoschizomers
BspC I, Ple19 I.
 
No. of Cleavage Sites
 
Note
Not blocked by dam methylation. Religation of DNA fragments with dam methylation is difficult.