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OUR SUPPLIERS

KOMABIOTECH
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Sacace Biotechnologies S.r.l.
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Spherotech, Inc.
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Exalpha Biologicals, Inc.
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SCETI K.K
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EY Laboratories, Inc. Headquarters
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EXBIO Praha, a.s.
 Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 
Human TNF (Tumor Necrosis Factor) alpha ELISA Kit
The Exalpha Biologicals, Inc. human Tumor Necrosis Factor alpha (TNFa) ELISA is a non-isotopic immunoassay for the in vitro quantitation of human phospho TNFa in serum, plasma, cell culture supernatants.

 

Features
- Colorimetric assay
- HTS compatible format
- High sensitivity
- 4 deg C storage with long shelf life
- Non-radioactive
- 5 hour protocol

 		 Performance Summary
- Sensitivity: 20 pg/ml
- Precision: <10% well-to-well variation
- Linearity: Within 5% of expected values
Ordering Information
Cat. No.
X1851K (pdf)
 		 Size
1 Plate
 
For larger quantity pricing, please contact Customer Service

Protocol Summary: Not to be used in place of detailed protocol. See kit manual for complete protocol.

Steps
Incubation
1. Equilibrate kit to room temperature -
2. Add samples and standards to wells 2 hours room temp. (or overnight 4°C)
3. Wash 4x -
4. Add detector antibody to all wells 2 hours - room temp.
5. Wash 4x -
6. Add HRP conjugate to all wells 30 minutes - room temp.
7. Wash 4x -
8. Add substrate to all wells 30 minutes - room temp.
9. Add stop solution to all wells -
10. Read plate at 450 nm/550 nm -