KOMABIOTECH
301, Gayang
Technotown,
#1487 Gayang 3
dong, Gangseo-gu
Seoul 157-793,
KOREA
Sacace
Biotechnologies
S.r.l.
Via Scalabrini,
44
22100 Como Italy
Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
Exalpha
Biologicals,
Inc.
2 Shaker
Road,
Unit
B101
Shirley,
MA 01464
SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN
EY
Laboratories, Inc.
Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA
EXBIO
Praha, a.s.
Nad
Safinou II 366
252 42 Vestec
Czech Republic
EzWay Antibody Biotin Labeling Kit
Catalog Number
K07900
Size
10T
Type
Kit
Datasheet
Background
Biotin is used in two-step detection systems in
concert with conjugated avidin. Biotin is
typically conjugated to proteins via primary
amines(i.e., lysines). Usually, between 3 and 6
biotin molecules are conjugated to each
antibody. The entire conjugation can be
performed in about an hour. In general, you will
need to have a solution of your antibody at a
concentration (optimally) of at least 2 mg/ml.
The extent of biotin conjugation to the antibody
may depend on the concentration of antibody in
solution; for consistent conjugations, use a
consistent concentration. This product provides
convenient and efficient method for removing
salt and amine compound interfering biotin
coupling reaction from antibody solution.
Features
Quick: only 1 hour to get conjugates
Easy: no filtration tube required
Reliable: high recovery & consistent
Convenient : removing salt and amine
compound interfering biotin coupling
reaction from antibody solution
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