SensiMix SYBR Low-ROX Kit


Features and Benefits Sensitivity: Optimized premixed with SYBR Green I and ROX, allows reproducible detection of low-copy number templates Speed: More efficient amplification results in earlier C(t)s and shorter overall real-time PCR reaction times without compromising performance Specific: Proprietary hot-start chemistry minimises non-specific amplification Broad dynamic range: As few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude Instrument Compatibility ABI 7500 and StepOneTM, Stratagene Mx4000®, Mx3000P® and Mx3005P®. (See product selection table). Description SensiMix Low-ROX kit is a high performance product designed for superior sensitivity and specificity on various real-time instruments in which a ROX passive reference signal is required, particularly for those block-based platforms which are more sensitive to the dye levels. The mix has been developed to provide the high sensitivity required for demanding assays or those assays where template is limited. Volume QT625-02: 250 x 50µl Reactions: 5 x 1.25ml QT625-05: 500 x 50µl Reactions: 10 x 1.25ml QT625-20: 2000 x 50µl Reactions: 40 x 1.25ml Components 2x Reaction mix consisting of: Heat-activated DNA polymerase Ultra-pure dNTPs MgCl₂ (6mM) Internal reference (low levels) SYBR® Green I Plus separate vials of: 50mM MgCl₂ Kit Size The kit size is based on a 50µl final reaction volume. Shipping Conditions 6 months at -20°C. Avoid exposure of the SYBR® Green I to light.