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Mbo II
Catalog Number K1750083
Size 100U
Type Buffer & Reagent
Datasheet Inquire
 
Recogntion Site
 
Concentration
5-15 units/ul. Assayed on unmethylated λ DNA.
 
Source
Moraxella bovis ATCC10900
 
Reaction Condition
Buffer B
[6mM Tris-HCl (pH7.5 @ 37°C), 6mM MgCl2, 50mM NaCl, 1mM DTT]
Incubate at 37°C
 
Storage Condition
  • 10mM Tris-HCl (pH7.4)
  • 50mM NaCl
  • 0.1mM EDTA
  • 1mM DTT
  • 0.5mg/ml BSA
  • 50% glycerol
  • Store at -20°C
 
No. of Cleavage Sites
 
Note
Blocked by overlapping dam methylation. Single base extension produced by Mbo II digestion is difficult to ligate.