EzWay TMB Substrate Kit (TMB+Substrate Solution)

[ Home ] [ Up ] [ Home ] [ Up ] EzWay TMB Substrate Kit (TMB+Substrate Solution) Home | ELISA Accessories | Detection Kits

Free Live Chat Software

GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

Tel + 32 16 58 90 45

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

Tel 01 43 25 01 50

Fax 01 43 25 01 60

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel 0241 56 00 99 68

Fax 0241 56 00 47 88

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719

Danmark

+4569918806

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494

Ireland Dublin
+35316526556

Luxembourg
+35220880274

Magyarország Budapest
+3619980547

Nederland
+31208080893

Norge Oslo
+4721031366

Österreich
+43720880899

Sverige Stockholm
+46852503438

Schweiz Züri
+41435006251

Northern America

Canada Montreal
+15149077481

US New York
+17185132983

Asia 日本
+81 78 386 0860

Other Countries
0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42 Vestec
Czech Republic

EzWay TMB Substrate Kit (TMB+Substrate Solution)

Catalog Number	K0331060
Size	1000well
Type	Buffer & Reagent
Datasheet

Specification

Most sensitive chromogenic substrate for peroxidase detection.
2 component system which develops a deep blue product when reacted with horseradish peroxidase (HRP) in ELISA.
TMB substrates contains 3,3', 5,5' - Tetramethylbenzidine.

Protocol

Dispense 150ul Color Reaction Mixture into each well (Or apply 50ul TMB solution and 100ul Substrate Solution to each well. Mix well by vortexing or shaking slightly).
After sufficient color development (5-10minutes at room temperature or at 37℃), add 50ul Stop Solution (2M H2SO4) to each well.
Read plates in a microwell plate reader at wavelength setting of 450nm.

Kit Contents

Items	Quantity
TMB Solution in organic base	60ml
Substrate Solution (H2O2 in an acidic buffer)	125ml