EzWay Protein-Blue I Staining Solution (for non-fixation)

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EzWay Protein-Blue I Staining Solution (for non-fixation)

Catalog Number	K14010
Size	500ml
Type	Buffer & Reagent
Datasheet	Inquire

Specification

Staining solution for non-fixation or protein recovery
Ready-to-use protein staining solution
Highly sensitive protein staining
Highly sharp protein band formation
Very low background
Water-based destaining
No need for multi-step washing
Staining of 2D gel

Protocol

Shake and mix the EzWay Protein-Blue I vigorously.
Wash the gel 3 times in 100 ml of D.W. for 10 minutes with gentle shaking.
Note: SDS interferes staining procedure, so wash thoroughly. It is not necessary for the native gel staining.
Add 20 ml of EzWay Protein-Blue I to stain a mini gel. Gently shake the tray.
Note: More staining solution may be required if a large tray or a large gel is being used.
Stain intensity reaches a maximum within approximately 1 hour. Gels may be stained overnight without increased background. Longer procedure is needed for thicker or higher percentage gel.
Wash the gel with D.W. Several D.W. changes may be necessary for optimal results.
Note: Washing overnight will reduce staining intensity.
After using EzWay Protein-Blue I, close tightly the cap and store again at 4°C.