EzWay pink-ONE TMB Substrate, 100ml

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EzWay pink-ONE TMB Substrate, 100ml

Catalog Number	K0331070
Size	1000well
Type	Buffer & Reagent
Datasheet

Specification

One-step prestained TMB
No mixing of TMB substrate and substrate soluton required
Pink-color coded indicator: Convenient to track for reagent supply
No interference of color to activity or sensitivity of reaction (H2O2 makes color disappeared)

Protocol

Dispense 100ul of pink-ONE TMB to each well. After sufficient color development (5-10 minutes at room temperature or at 37°C), add 100 ul Stop Solution (2M H2SO4) to each well.
Read plates in a microwell plate reader at wavelength setting of 450nm.