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Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
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EzWay Opti-PCR Kit
Specification
A simple way to determine Optimal Buffer
Conditions for PCR
Efficiency and specificity of amplification highly depends on the nature of template DNA and primers. Therefore, the optimization of reaction conditions to obtain satisfactory results is necessary because of a large number of variables such as pH, concentrations of salt and magnesium ions etc. KOMA EzWayTM Opti-PCR is designed for supplying the simple procedure to optimize PCR conditions which may take much time and effort. The ready-to-use reaction buffers will cover a wide range of possible values for all ingredients. Furthermore, the Magic buffer supplied in this kit will be helpful to obtain the sufficient specificity of amplification of high GC template DNA. Bisides of 15 different Opti-PCR buffers (5,10,15,20,25mM MgCl2 based buffers at pH8.3, 8.7, 9.0 respectively), the most popular additives and cosolvents such as BSA, formamide, dimethyl sulfoxide (DMSO) and glycerol are also included in the kit as well.
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