EzWay Buccal Swab DNA Isolation Kit

EzWay Buccal Swab DNA Isolation Kit

Catalog Number	K33305	K33310
Size	50T	100T
Type	Kit	kit
Datasheet

Specification

EzWay™ Buccal Swab DNA Isolation kit is a simple, fast and straightforward method for isolating buccal cell genomic DNA from buccal swab sample. Buccal swabs are the least invasive way of collecting gDNA from human, and also have the added benefit of minimizing the exposure to blood-borne pathogens. Cells collected on buccal swabs do not require special storage conditions and the DNA remains useable after years of storage. Due to the high purity, the isolated genomic DNA is ready to use for a broad panel of downstream applications such as PCR. This kit has been validated for use with several types of swabs, and will most likely work with others.

Easy-to-Use protocol for isolation of genomic DNA from Buccal swab sample
High yield and purity of isolated genomic DNA
Time saving procedure, just 20 min for total procedure

Kit Contents

Lysis Buffer
Binding Buffer
Wash Buffer (concentrated)
Elution Buffer
Dilution Buffer
Spin Columns with Collection Tubes
Proteinase K

Protocol

Applications

Spectrophotometric data for the genomic DNA isolated from buccal swab samples is given in Table 1.
Genomic DNA was analyzed by agarose gel electrophoresis (Figure 1). Undigested DNA was resolved on a 1% agarose gel and visualized by ethidium bromide staining. As shown in Figure 2, PCR amplification of GAPDH gene using control primer was done successfully. Yields of DNA from swabs are highly variable depending on the swab type, the person being swabbed, the swabbing technique, and the number of cells captured on the swab. The expected yield using this protocol is 300 ng to 3 ug per swab.

Table 1. Genomic DNA isolation from buccal swabs. Cotton swabs were used on twelve different donors. Yields in this study were measured using a NanoDrop® ND-1000 instrument.

Figure 1. Isolated genomic DNA
4 ul of each isolated template from buccal swab was resolved on a 1% agarose gel and visualized by ethidium bromide staining.

Figure 2. PCR amplified of GAPDH gene
4 ul of each PCR product using control primer was resolved on a 1.2% agarose gel and visualized by ethidium bromide staining.