KOMABIOTECH
301, Gayang
Technotown,
#1487 Gayang 3
dong, Gangseo-gu
Seoul 157-793,
KOREA
Sacace
Biotechnologies
S.r.l.
Via Scalabrini,
44
22100 Como Italy
Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
Exalpha
Biologicals,
Inc.
2 Shaker
Road,
Unit
B101
Shirley,
MA 01464
SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN
EY
Laboratories, Inc.
Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA
EXBIO
Praha, a.s.
Nad
Safinou II 366
252 42 Vestec
Czech Republic
EzWay Annexin V-FITC Apoptosis Detection Kit
Catalog Number
K29100
Size
100T
Type
Kit
Datasheet
Features
Utilizes FITC-conjugated Annexin V
protein for detection of cells undergoing
apoptosis
Annexin V FITC binds to the membranes of
apoptotic cells
Detection by flow cytometry or
fluorescence microscopy
Reading of Result
Non-apoptotic cells: Annexin-V negative
and PI negative
Non-apoptotic cells: Annexin-V negative
and PI negative
Necrotic cells or late apoptotic cells:
Annexin-V positive and PI positive
Kit Contents
Annexin V-FITC
5X Binding buffer
5X Binding buffer
How to Use
Cell preparation and rinsing cells with
Binding Buffer
Add Annexin V-FITC
Add Propidium Iodide
Incubate at 4℃ in the dark.
Detection
Staining of healthy and apoptotic
Jurkat cells using flow cytometry
Jurkat cells were incubated with 0.5ug/ml
actinomycin D for 20 hours. Untreated cells
are shown in the left panel, apoptotic
induced cells are shown in the right panel.
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