Direct Mouse Tail PCR

EzWay™ Mouse Tail Direct PCR Kit

Catalog Number	K0568600
Size	1set
Type	Kit
Datasheet

Description

KOMA Biotech launched its new EzWay™ Mouse Tail Direct PCR Kit, which is designed to enable DNA amplification directly from mouse tail without DNA extraction. Just cut the mouse tail and incubate it in the lysis buffer provided in the kit for 10 minutes and then use 1ul of lysate for PCR. It doesn't require overnight incubation or Proteinase K (PK) treatment as well as DNA purification process. Direct Hot Taq PCR MasterMix in this kit is a convenient mixture of Taq enzyme, dNTP, MgCl2, dye, additives and Direct PCR Buffer, so you can just add the tail lysate and primers, and then go for PCR. This kit will save your enormous amounts of material, cost and time from conventional genotyping methods. Also, it reduces risk of handling errors and contamination.

Features

Genotyping of transgenic mouse
Fast and simple method for genotyping without DNA purification
Just 10 minutes incubation required
No need to add Proteinase K
Saving enormous amounts of material and time
Optimized MasterMix type containing Taq enzyme, dNTP, Direct PCR Buffer, MgCl2, Red dye and additives
Direct loading of PCR products without adding red dye

Kit Contents

Direct Hot Taq PCR MasterMix	1ml
5X Direct Lysis Buffer	1ml
Magic Buffer	1.5ml

How to use

Cut mouse tail 1-2mm length and add lysis buffer.
Incubate at 60°C for 10 minutes
Vortex and spin down briefly.
Use 1ul supernatant of lysate and go to PCR directly.

Direct PCR from mouse tail tissue :

20ul PCR reactions with 2, 0.2, 0.04 and 0.02ul concentrations of 10mg mouse tail lysed with lysis buffer were performed using the recommended protocol with and without PK treatment.

Lane 1,3,5,7 : Direct PCR after lysis using Proteinase K at 56°C for 10 min and 95°C for 5 min
Lane 2,4,6,8: Direct PCR without PK treatment. Incubation at 60°C for 10 min.