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APO ACTIVE 3 ™
Antibody Specific Active Caspase 3 Detection Kit
 
Key Benefits:
  • Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
  • Applications  - Works with a fluoresence microscope, 96 well plate reader, or flow cytometer.
  • Yields both quantitative and qualitative results. Gives a strong positive signal.
  • Can be used in conjunction with other antibodies or stains.
  • No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
Assay Principle
Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.
Kit contents
  • 1 vial of Rabbit anti cleaved caspase 3 affinity purified polyclonal antibody
  • 1 vial of FITC labeled Goat anti Rabbit affinity purified polyclonal antibody
  • 1 vial of 10X fixative.
The following kits are available:
Product
 		 Catalog No.
 		 Size
(No. of Tests)		 Price (Eur)
APO ACTIVE 3 Antibody Specific Active Caspase 3 Detection Kit FAB200-1 25 €145
FAB200-2
 		 100 €425

(A)

(B)
Jurkat Cells were stimulated with staurosporine (B) or DMSO (A) for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti Rabbit antibody.