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Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
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EzWay Antibody Biotin Labeling Kit
Background
Biotin is used in two-step detection systems in concert
with conjugated avidin. Biotin is typically conjugated
to proteins via primary amines(i.e., lysines). Usually,
between 3 and 6 biotin molecules are conjugated to each
antibody. The entire conjugation can be performed in
about an hour. In general, you will need to have a
solution of your antibody at a concentration (optimally)
of at least 2 mg/ml.
The extent of biotin conjugation to the antibody may depend on the concentration of antibody in solution; for consistent conjugations, use a consistent concentration. This product provides convenient and efficient method for removing salt and amine compound interfering biotin coupling reaction from antibody solution.
Features
Kit Contents
How to Use
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