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Tris-Tricine-SDS PAGE Buffer (10X)

 
 
  • Running Buffer for Small Proteins
  • Variation on the Schagger/von Jagow System
  • Substitute for Tris-Glycine-SDS in the Standard Laemmli Protocol
Discontinuous SDS-PAGE employing Tris-Glycine as the tank buffer resolves proteins down to about 15 kd. However, below this size, the proteins do not destack from the SDS micelles running through the gel with the buffer front. In order to resolve proteins in this size range, the Tris-Tricine system of Schagger and von Jagow (1987) was developed.
An Alternative to the Schagger and von Jagow System involves running Tris-Tricine Gels using National Diagnostics 10X Tris-Tricine-SDS Buffer (EC-869). With National Diagnostics Tris-Tricine-SDS, you can extend the range of SDS-PAGE to resolve smaller proteins with minimal alteration of protocol.
To provide this level of convenience, National Diagnostics streamlined the original method of Shaggar and Von Jagow (1987 Anal. Biochem 166) by developing Tris-Tricine-SDS cathode tank buffer to be compatible with the standard Laemmli gel/buffer system. This combination resolves proteins as small as 5kD. The researcher simply substitutes National Diagnostics Tris-Tricine-SDS in the upper (cathode) tank, with no other changes from standard Laemmli protocol to extend the resolution of their gels.

Catalog Number: EC-869
 

APPLICATIONS OF TRIS-TRICINE-SDS PAGE BUFFER (10X)
Electrophoresis Buffers
Denaturing Protein Electrophoresis: SDS-PAGE
Related Products   
ProtoGel Quick-Cast 12%
Ready-to-use gel solution for SDS-PAGE that combines the convenience of pre-cast gels with the economy of casting your own.
 
ProtoGel (30%)
30% concentrated solution of acrylamide and bis-acrylamide, 37.5 : 1 ratio. Filtered, deionized, and stabilized.
 
ProtoGel (40%)
Concentrated solution of acrylamide and bis-acrylamide, 37.5 : 1 ratio. Filtered, deionized, and stabilized.
 
AccuGel 29:1
Concentrated solution of acrylamide and bis-acrylamide (29:1) for native DNA electrophoresis and SDS-PAGE. Filtered, deionized, and stabilized.
 
Tris-Glycine-SDS PAGE Buffer (10X)
Ultra-pure concentrated standard running buffer for SDS-PAGE. Formulated with 18 MegOhm water. 0.2 micron filtration.
 
ProtoGel Resolving Buffer (4X)
4X Laemmli Tris-HCl resolving gel buffer for clear, reproducible SDS-PAGE.
 
ProtoGel Stacking Buffer
4X Laemmli Tris-HCl stacking gel buffer for clear, reproducible SDS-PAGE.
 
Protein Loading Buffer Blue (2X)
Ready to use sample buffer for SDS-PAGE.
 
Protein Loading Buffer Blue (2X)
Ready to use sample buffer for SDS-PAGE.
 
ProtoBlue Safe
Eco-friendly, ultra-sensitive colloidal coomassie G-250 stain.
 
ProtoMarkers
Broad range pre-stained protein markers for SDS-PAGE.
 
Ammonium Persulfate - ULTRA PURE
Exceeds ACS standards. Low absorbed water results in consistent initiation.
 
TEMED - ULTRA PURE
Fractionally distilled to remove all trace metals and amine impurities. Stored under nitrogen.