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Taq DNA Polymerase Kit I and Taq DNA Polymerase Kit II (MgCl2 Free Reaction Buffer)
Catalog Number K0561001 K0561002 K0561003 K0561004 K0561005 K0561006
Size 250U 500U 1000U 250U 500U 1000U
Type PCR Kit PCR Kit PCR Kit PCR Kit PCR Kit PCR Kit
Datasheet
 
Description
Taq DNA Polymerase is the enzyme from the cloned Thermus aquaticus expressed in E.coli. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium and also possesses a 5'→3' exonuclease activity.
Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
 
Features
  • Ultrapure, thermostable recombinant enzyme
  • Highly thermostable: 10min at 97°C, 60min at 94°C
  • 5'→3' exonuclease activity
  • Extra A addition activity
  • Amplification range:
    • Lambda DNA: up to 6Kb (Max. 12Kb)
    • Human genomic DNA: up to 2Kb (Max. 4Kb)
  • Composition of Taq polymerase and Taq PCR buffer containing MgCl2
  • No Nuclease, RNase, Protease contamination
 
Applications
  • Amplification of genomic DNA and cDNA targets up to 3kb long
  • GC-rich sequences or secondary structures
  • TA Cloning
  • Differential Display
  • Degenerate PCR
 
Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTPs into an acid-insoluble product in 30 minutes at 74°C.
 
Source
E. coli, recombinant gene from Thermus aquaticus
 
Packing
Component Cat.No
Taq DNA Polymerase I, 250U K0561001
10X Taq PCR Buffer I (with MgCl2) K0561011
dNTP Mix (2.5mM each) K1756011
10X Loading Buffer K0561051
Nuclease Free Water K0561061
4X Magic Buffer (for high GC content) K0561031
 
 
PCR for human beta-globin gene (A) and lambda DNA (B) were performed using a serial dilution of Taq DNA Polymerase.
 
The Magic buffer is very useful to amplify DNA of high GC contents or of complicated structure. Human retinoblastoma gene (A), human apolipoprotein E gene (B) and human Htra serine protease1 gene (C) were amplified by addition of Magic buffer to Taq DNA Polymerase.