Home ] Up ] Call me back Contents Immunology Infectious agents Cellular Biology Molecular biology Instruments Chimical Product PCR ELISA Sites Komabiotech products ProductHome ] Up ] Superoxide Dismutase (SOD) Detection Kit Home | Superoxide Dismutase (SOD) Detection Kit | Nitric Oxide Synthase Detection Kit | Hydroxyl (OH)/ Peroxynitrite (ONOO-)Detection Kit | Fluoro H202

Google

       Pricelist 2010 

Home
Up

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

Superoxide Dismutase (SOD) Detection Kit
Colorimetric  Detection Kit
Key Benefits:
 
  • 100% Inhibition by Super Oxide Dismutase (SOD)
  • Can detect low concentrations of SOD
  • Highly water-soluble formazan dye.
  • Applications: colorimetric detection.
Introduction:
       Superoxide dismutase (SOD) are metalloenzymes that catalyze the dismutation of superoxide radical into hydrogen peroxide (H2O2) + molecular oxygen (O2) and consequently provide an important defense mechanism against superoxide radical toxicity (1).
       Oxidative stress dependent upon superoxide radical can account for a number of acute and chronic disease states, which include inflammation and ischemia-reperfusion (2,3). SOD protects murine peritoneal macrophages from apoptosis induces by adriamycine (4). Furthermore, over expression of SOD in fibrosarcome cells, protects against apoptosis and promotes cell differentiation (5). 
Assay Principle:
To determine SOD activity, several direct and indirect methods have been developed. A common and convenient indirect method utilizes nitroblue tetrazolium (NBT) conversion to NBT-diformazan (formazan dye) via superoxide radical. However, there are several disadvantages to the NBT method, such as poor water solubility of the formazan dye and the interaction with the reduced form of xanthine oxidase. Though cytochrome C is also commonly used for SOD activity detection, its reactivity with superoxide is too high to determine low levels of SOD activity.

Cell Technology’s SOD kit utilizes a water-soluble tetrazolium salt, WST-1 (2-(4-Iodophenyl)- 3-(4-nitrophenyl)-5-(2,4-disulfophenyl)- 2H-tetrazolium, monosodium salt) that produces a highly water-soluble formazan dye upon reduction with a superoxide anion (6). The rate of the reduction with O2.- is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD, as shown in Figure 1. Therefore, the IC 50 (50% inhibition activity of SOD or SOD-like materials) can be determined by this colorimetric method. Absorbance can be measured at 440nm.

SOD Inhibition Curve

Figure 1 - Inhibition Curve Prepared Using SOD from Bovine Liver

SOD Assay Kit-WST

Figure 2 -  SOD ASSAY Reaction

The following kits are available:
Catalog 
 
Contents Size Price
CSOD 100-2
 
See Below* 100 Tests  €225