Home ] Up ] Call me back Contents Immunology Infectious agents Cellular Biology Molecular biology Instruments Chimical Product PCR ELISA Sites Komabiotech products ProductHome ] Up ] SensiMix SYBR Kit Home

Google

       Pricelist 2010 

Home
Up

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

SensiMix SYBR Kit
 
  Cat No. Size Conc.  
  QT605-02 2x 250 Reactions  
  QT605-05 2x 500 Reactions  
  QT605-20 2x 2000 Reactions  
 
Please contact info@bioline.com for Bulk, Custom & OEM services.
 

 
 
 
Features and Benefits

 
  • Simple and reproducible: a choice of optimized kits for different platforms, just add primers and template, reducing possible errors in set-up
  • Specific and sensitive: for detection of a wide range of template concentration, even from hard to obtain and low copy number samples
  • Fast protocols: can be used with the new generation of fast cyclers without compromising performance
  • Flexible: validated with all major real-time instruments
  • 2x master mix: premixed with SYBR Green I and an internal reference for convenience


 
Instrument Compatibility

ABI 7000, 7300, 7700, 7900, 7900HT and StepOne™. (See product selection table)


 
Description

Versatility
  • Mix optimised for SYBR® Green I chemistry.
  • Suitable for high throughput applications e.g. target validation for drug discovery.
  • Designed to work across a range of block and rotor-based instruments.

SensiMix SYBR kit contains all the components necessary to perform consistent and reproducible real-time PCR assays with your DNA template. The kits deliver highly specific and sensitive results across a wide dynamic range of template concentrations (over 10 fold), producing reliable real-time data quickly and easily. The kits are suitable for DNA templates or RNA templates following reverse transcription with the Bioline cDNA Synthesis Kit (BIO-65025) and are compatible with the ABI 7000, 7700, 7900HT and StepOne™ platforms. (See product selection table)

The kits are powered by SensiTaq, a highly-sensitive hot-start DNA polymerase that provides higher specificity, and ROX passive reference fluorescent dye, making it specifically suitable for use on real-time instruments that measure this passive reference signal.


 
Volume

QT605-02: 250 x 50µl Reactions: 5 x 1.25ml
QT605-05: 500 x 50µl Reactions: 10 x 1.25ml
QT605-20: 2000 x 50µl Reactions: 40 x 1.25ml


 
Components

2x Reaction mix consisting of:
  • Heat-activated DNA polymerase
  • Ultra-pure dNTPs
  • MgCl2 (6mM)
  • Internal reference
  • SYBR® Green I


 
Kit Size

The kit size is based on a 50µl final reaction volume.


 
Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.


 
Product Citations

1. Lappas M., et al. Placenta 30(3): 256-262 (2009)
2. Rosker C., et al. J. Biol. Chem. 284(8): 5186-5194 (2009)
3. Barker H.E, et al. BMC Developmental Biology 8(1): 94 (2008)
4. Johnson C.E. et al. StemCells 25: 1913–1923 (2007)


 
.
“What was a problem before now works perfectly! I tried several SYBR Green Kits but SensiMix SYBR worked best. Triplicates have very good standard deviations and detection of even low transcript levels via real-time RT-PCR are no problem anymore in Plasmodium falciparum.”

Dipl. biol. Anne Röseler, Nutritional Biochemistry, Justus-Liebig University Giessen, Germany.


 
 
   
  Product Images
 
 

Click image to enlarge