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SensiMix SYBR & Fluorescein One-Step Kit
 
  Cat No. Size Conc.  
  QT265-01 2x 25 Reactions  
  QT265-05 2x 500 Reactions  
  QT265-20 2x 2000 Reactions  
  QT265-02 2x 250 Reactions  
 
 
Features and Benefits

 
  • Flexible: suitable for block-based instruments where fluorescein is required to allow calculation of dynamic well factors
  • Reproducible: master-mix contains reverse transcriptase for efficient cDNA synthesis together with an optimized amount of highly engineered hot-start SensiTaq enzyme, to provide reproducible real-time data
  • Sensitive: all template material is used in the reaction, which is especially useful when amplifying a low copy number target from limited starting material, allowing as few as 10 copies of a target gene to be detected
  • Convenient: reduced pipetting steps and possible contamination and RNA degradation


 
Instrument Compatibility

Bio-Rad iCycler®, iQTM5 and MyiQ™. (See product selection table)


 
Description

The SensiMix SYBR & Fluorescein One-Step Kit provides optimal performance and maximum convenience for real-time, quantitative analysis of RNA templates in a single reaction format on real-time instruments that measure a fluorescein passive reference channel, such as the Bio-Rad iCycler®, iQ™5 and MyiQ™ platforms. As few as 10 copies of a target gene can be detected using the SensiMix SYBR & Fluorescein One-Step Kit.


 
Volume

QT265-01: 25 x 50µl Reactions: 1 x 625µl
QT265-02: 250 x 50µl Reactions: 5 x 1.25ml
QT265-05: 500 x 50µl Reactions: 10 x 1.25ml
QT265-20: 2000 x 50µl Reactions: 40 x 1.25ml


 
Components

2x Reaction mix consisting of:

 
  • Heat-activated DNA polymerase
  • Sensi reverse transcriptase
  • Internal reference
  • Ultra-pure dNTPs
  • MgCl2 (6mM)
  • SYBR® Green I


Plus separate vials of:

 
  • 50mM MgCl2
  • RNase inhibitor
  • DEPC-treated water


 
Kit Size

The kit size is based on a 50µl final reaction volume.


 
Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.


 
 
   
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