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SensiMix SYBR & Fluorescein Kit
 
  Cat No. Size Conc.  
  QT615-20 2x 2000 Reactions  
  QT615-02 2x 250 Reactions  
  QT615-05 2x 500 Reactions  
 
Please contact info@bioline.com for Bulk, Custom & OEM services.
 

 
 
 
Features and Benefits

 
  • Simple and reproducible: premixed with SYBR Green I and fluorescein, just add primers and template, reducing errors in set-up
  • Specific and sensitive: for detection of a wide range of template concentration, even from hard to obtain and low copy number samples
  • Fast protocols: can be used with the new generation of fast cyclers without compromising performance
  • Flexible: suitable for block-based instruments where fluorescein is required to allow calculation of dynamic well factors


 
Instrument Compatibility

Bio-Rad iCycler®, iQTM5 and MyiQ™. (See product selection table)


 
Description
The SensiMix SYBR & Fluorescein Kit contains fluorescein, making it specifically suitable for use on real-time instruments that measure fluorescein passive signal, such as the Bio-Rad iCycler®, iQ™5 and MyiQ™ platforms. Both SYBR® Green I and fluorescein are premixed for immediate use.

With SYBR® Green I and fluorescein in their premixed and optimized concentrations, as few as 10 copies of a target gene can be detected


 
Volume

QT615-02: 250 x 50µl Reactions: 5 x 1.25ml
QT615-05: 500 x 50µl Reactions: 10 x 1.25ml
QT615-20: 2000 x 50µl Reactions: 40 x 1.25ml


 
Components

2x Reaction mix consisting of:

 
  • Heat-activated DNA polymerase
  • Ultra-pure dNTPs
  • MgCl2 (6mM)
  • Fluorescein
  • SYBR® Green I


Plus separate vials of:
 
  • 50mM MgCl2


 
Kit Size

The kit size is based on a 50µl final reaction volume.


 
Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.


 
Product Citations

1. Dhawan R. et al. The Plant Cell 21: 1000-1019 (2009)
2. Maatouk D.M. et al. Human Molecular Genetics 17(19): 2949-2955 (2008)
3. Del Rosario B.C. & Pemberton L.F. Molecular and Cellular Biology 28(7): 2113-2124 (2008)
4. Miller M.R. et al. J. Nutr. 138(11):2179-2185 (2008)


 
 
   
 
 
  Product Images
 
 

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