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SensiMix Capillary One-Step Kit
 
  Cat No. Size Conc.  
  QT255-02 2x 250 Reactions  
  QT255-20 2x 2000 Reactions  
  QT255-01 2x 25 Reactions  
  QT255-05 2x 500 Reactions  
 
Please contact info@bioline.com for Bulk, Custom & OEM services.
 

 
 
 
Features and Benefits

 
  • Simple: developed for both SYBR Green I and probe assays
  • Specific: optimised buffer specifically designed for real-time instruments that use glass capillaries
  • Reproducible:master-mix contains reverse transcriptase for efficient cDNA synthesis together with an optimized amount of highly engineered hot-start SensiTaq enzyme, to provide reproducible real-time data
  • Sensitive: accurate linear standard curve from a 10-fold RNA dilution series over a broad dynamic range
  • Convenient: reduced pipetting steps and possible contamination and RNA degradation


 
Instrument Compatibility

Roche LightCycler® 1.0 and 2.0(See product selection table).


 
Description

The SensiMix Capillary One-Step Kit has been developed to perform highly reproducible cDNA synthesis and subsequent real-time PCR assays in one tube, on glass capillary-based real-time instruments such as the Roche LightCycler® 1.0 and 2.0 platforms (See product selection table). The kit contains BSA to ensure compatibility with capillary based machines, along with reverse transcriptase for efficient cDNA synthesis together with an optimized amount of highly engineered hotstart SensiTaq enzyme, allowing highly reproducible first strand cDNA synthesis and subsequent real-time PCR assays in one tube.

The kit has been developed to provide the high sensitivity required for challenging assays on capillary instruments both for probe and for SYBR® Green I based chemistries. In order to reduce degradation of RNA, a separate tube of RiboSafe RNase inhibitor is included to minimize enzymatic degradation of the RNA by contaminating RNase activity.


 
Volume

QT255-01: 25 x 20µl Reactions: 1 x 1.250µl
QT255-02: 250 x 20µl Reactions: 2 x 1.25ml
QT255-05: 500 x 20µl Reactions: 4 x 1.25ml
QT255-20: 2000 x 20µl Reactions: 16 x 1.25ml


 
Components

2x Reaction mix consisting of:

 
  • Heat-activated DNA polymerase
  • Sensi reverse transcriptase
  • Internal reference
  • Ultra-pure dNTPs
  • MgCl2 (6mM)


Plus separate vials of:

 
  • 50mM MgCl2
  • RNase inhibitor
  • DEPC-treated water
  • SYBR® Green I


 
Kit Size

The kit size is based on a 20µl final reaction volume.


 
Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.


 
 
   
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