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redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

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Österreich
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Schweiz Züri
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Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

Sample Diluents
Improves Assays:
By accurately reading and quantitating high-titer samples. High-titer samples may overload the finite binding capacity of the coated ELISA plate surface, thus requiring dilution prior to testing in the assay.
Diluents Available:
  • ESD100 General-Purpose Sample Diluent - All ELISAs For dilution of most serum, ascites, cell-culture supernatants, and antigens into the functional range of the ELISA.
  • ESD200 Plasma Sample Diluent - Antigen-Down For dilution of plasma samples into the functional range of the antigen-down ELISA to inhibit complement and thrombin (clotting) activity during the assay.
  • ESD300 Picses Sample Diluent.
Directions:
Simply multiply the value determined from the curve by your dilution factor. For example, if you diluted your sample 1:2 (250uL sample into 250uL sample diluent) and this yielded an OD of 0.437, when read off the curve, the OD of 0.437 correlated to 200 pg/mL, you would simply multiply 200pg/mL by 2 (your dilution factor) yielding a value of 400pg/mL, meaning that the original starting sample had 400pg/mL of analyte present.
ESD100 General-Purpose Sample Diluent - All ELISAs
General-Purpose Sample Diluent is formulated for diluting goat, rabbit, mouse, and human sera, as well as cell culture media and mouse ascites fluid. ESD100 can also be used for dilution of mammalian and chicken serum samples in antigen-down ELISA formats. Hyperimmunized serum samples contain nonspecific IgG antibody that may interfere with the titration. Proprietary additives have been incorporated into this diluent that minimize nonspecific binding interactions of the non specific IgG in the samples, thus decreasing background noise. Dilutions of the antigen standard for use in sandwich ELISA formats have also been carried out using this diluent.

ESD100 contains a buffered BSA protein base to assure a constant pH and solute environment favorable to antibody-antigen interactions. It also contains an antimicrobial agent to provide a solution free of bacterial contamination.
 
General-Purpose Sample Diluent (ESD100)
Catalog Number
 
Size (mL)
 
Samples diluted 1:2 using 250uL
 
Samples diluted 1:10 using 450uL Price
ESD100-1 100 400 220 €55.00
ESD100-2 500 2,000 1,100 €165.00
ECD100-3 1,000 40,00 2,200 €265.00
ECD100-4 10,000 40,000 22,200 €2,100.00
ESD200 Plasma Sample Diluent - Antigen-Down
Plasma Sample Diluent is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This diluent contains proprietary additives in a buffered BSA protein base with which provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. Additional additives have been included to inhibit thrombin (clotting) and complement activity during the plasma sample incubation period. ESD200 also contains an antimicrobial agent to provide a solution free of bacterial contamination.
Plasma Sample Diluent - Antigen-Down (ESD200)
Catalog Number
 
Size (mL)
 
Samples diluted 1:2 using 250uL
 
Samples diluted 1:10 using 450uL Price
ESD200-1 100 400 220 €75.00
ESD200-2 500 2,000 1,100 €230.00
ECD200-3 1,000 40,00 2,200 €365.00
ECD200-4 10,000 40,000 22,200 €2,900.00
ESD300 Nonmammilian Sample Diluent
Nonmammilian Sample Diluent contains proprietary additives in a buffered BSA protein base with which provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. ESD300 also contains an antimicrobial agent to provide a solution free of bacterial contamination.
Pisces Sample Diluent - (ESD300)
Catalog Number
 
Size (mL)
 
Samples diluted 1:2 using 250uL
 
Samples diluted 1:10 using 450uL Price
ESD300-1 100 400 220 €75.00
ESD300-2 500 2,000 1,100 €230.00
ECD300-3 1,000 40,00 2,200 €365.00
ECD300-4 10,000 40,000 22,200 €2,900.00