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Human ELISA kits         Mouse ELISA kits

Rat ELISA kits              Porcine ELISA kits

Product: RayBio® ELISA kits
      Introduction
  Highlight
  How it works
  Contents of kit
  Product List
  Service
  Reference List 
 
 
 
Introduction
The RayBio® Human IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human, mouse and rat protein level in serum, plasma, cell culture supernate, cell lysate, tissue lysate and urine. This assay employs a specific capture antibody coated on a 96-well plate. Standards and samples are pipetted into the wells and target protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
 
Highlight
The best products for confirmation of antibody array data
Complete kit with pre-coated 96 well plate
Highly sensitive and specific
Reproducible and reliable
Cost-effective
Optimized for serum, plasma, urine and conditioned medium
Hundreds of choices
 
How it works

Contents of kit
96-wells (12stripsx8 wells) pre-coated Microplate (Item A)
Wash Buffer Concentrate (20x) (Item B)
Standards (Item C)
Sample Diluent Buffer (Item D)
Assay Diluent (Item E)
Detection Antibody (Item F)
HRP-Streptavidin concentrate (Item G)
TMB One-Step Substrate Reagent (Item H)
Stop Solution (Item I)
Cell Lysis Buffer (for cell lysate or tissue lysate kit only) (Item J)
 
Representative Results
 
 
Reference List
 
1. Jean A. Nemzek, Javed Siddiqui, Daniel G. Remick: Development and optimization of cytokine ELISAs using commercial antibody pairs. Journal of Immunological Methods, 2001; 255:149–157.
2. Zhenyu Ju, Hong Jiang, Maike Jaworski, Chozhavendan Rathinam, Anne Gompf, Christoph Klein, Andreas Trumpp & K Lenhard Rudolph: Telomere dysfunction induces environmental alterations limiting hematopoietic stem cell function and Engraftment. Nature Medicine, 2007; 13: 742-747.
3. Anyuan Sun, Haiming Wei, Rui Sun, Weihua Xiao,Yongguang Yang, and Zhigang Tian: Human Interleukin-15 Improves Engraftment of Human T Cells in NOD-SCID Mice. Clinical and Vaccine Immunology, 2006; 13 (2): 227–234.
4. Jianping Xie, Hongyi Pan,Seungmin Yoo, and Shou-Jiang Gao: Kaposi’s Sarcoma-Associated Herpesvirus Induction of AP-1 and Interleukin 6 during Primary Infection Mediated by Multiple Mitogen-Activated Protein Kinase Pathways. Journal of Virology, 2005; 79 (24):15027–15037.
5. Mustafa Kerem, Zafer Ferahkose, Utku Tonguc Yilmaz, Hatice Pasaoglu, Ebru Ofluoglu, Abdulkadir Bedirli, Bulent Salman, Tevfik Tolga Sahin, Murat Akin: Adipokines and ghrelin in gastric cancer cachexia. World J Gastroenterol, 2008; 14(23): 3633-3641.
6. Ruochun Huang, Shiyong Li, Weimin Yang, Lipai Chen and Ruo-Pan Huang: Early Growth Response-1 Suppresses Human Fibrosarcoma Cell Invasion and Angiogenesis. CANCER GENOMICS & PROTEOMICS, 2006; 3: 71-82.