27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
2-10 units/ul. Assayed on λ DNA
[6mM Tris-HCl (pH7.9 @ 37°C), 6mM MgCl2, 150mM NaCl, 1mM DTT]
Incubate at 37°C.
BspC I, Ple19 I.
No. of Cleavage Sites
Not blocked by dam methylation. Religation of DNA fragments with dam methylation is difficult.