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redcoon België GENTAUR BVBA

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Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

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GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

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GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

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23015 Milano, Italy

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OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

 Universal Plate Coating Buffer
Improves Assays:
  • Saves valuable reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when this buffer is used.
  • Increases the shelf-life of your plates. Depending on the activity of the coated protein, plates may be stored at room temperature or 2°-8°C for several months, or even years under proper conditions.
  • Increases reproducibility. By using consistent reagents from a reliable source, your ELISAs may become more reproducible.
  • Increases the signal of the ELISA. If the antibody or antigen is not sticking to the plate, or unwanted proteins are interfering with binding, the plate will have an overall low specific signal. CB1 may foster adsorption and reduce interference.
  • Decreases background noise. If excess proteins are sticking to the plate, plate will have overall high backgrounds (a high signal overall, but a low specific signal). Universal Plate Coating Buffer may reduce interference.
How it Works:
Our Coating Buffer is an optimal formulation of buffers and salts which maximizes the adsorption of antibodies and antigens onto polystyrene plates. During the adsorption process, the Coating Buffer stabilizes coated proteins by maintaining their tertiary three-dimensional structure. By stabilizing the adsorbed protein, antigenic regions are preserved, allowing for greater binding reactivity with the detection molecule, thereby enhancing the specific signal. By generating a higher specific signal, a lower concentration of coat protein may be needed when this buffer is used, thereby saving valuable reagents.
Directions:
Simply dilute Coating Buffer 1:5 (for example, add 100mL Coating Buffer to 400mL diH20 for a final volume of 500mL) , add your protein antigen or antibody, let the solution stir for 15 minutes, and pipette onto the plate.
ECB100 Universal Plate Coating Buffer
Cell Technology has specifically formulated a unique plate coating buffer for use with antibodies and antigens. The pH and ionic strength of this buffer have been specifically formulated to create an optimal coating environment which maximizes the adsorption efficiency of most antibodies and protein antigens onto polystyrene plate surfaces. This buffer also stabilizes coated proteins, preserving antigenic regions, and allowing for greater binding reactivity with the detection molecule, enhancing the specific signal. By generating a higher specific signal, a lower concentration of coat protein may be needed when this buffer is used, thereby saving valuable reagents.
5x Universal Plate Coating Buffer (ECB100)
5x Bottle Size
 
Price per Bottle
 
Part Number
 
Volume at 1x Plates at 50uL/well
(4.8mL/plate)
Plates at 200uL/well
(9.6mL/plate)
Plates at 300uL/well
(28.8mL/plate)
100 mL
 
€40 644
 
500mL
 
100
 
25
 
16
500 mL
 
€95 645
 
2.5L
 
510 125 84
1 L €195.00 646
 
5L 1020
 
250 170
10 L €1350.00 658 50L 10,200
 
2,500 1,700