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OUR SUPPLIERS
KOMABIOTECH
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Sacace
Biotechnologies
S.r.l.
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Spherotech, Inc.
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Exalpha
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EY
Laboratories, Inc.
Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA
EXBIO
Praha, a.s.
Nad
Safinou II 366
252 42 Vestec
Czech Republic
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Universal Plate
Coating Buffer |
Improves Assays: |
-
Saves valuable reagents. By promoting a higher
specific signal, less protein may be needed to coat the plate
and less of the detection molecules may be needed to generate a
signal when this buffer is used.
-
Increases the shelf-life of your plates.
Depending on the activity of the coated protein, plates may be
stored at room temperature or 2°-8°C for several months, or even
years under proper conditions.
-
Increases reproducibility. By using consistent
reagents from a reliable source, your ELISAs may become more
reproducible.
-
Increases the signal of the ELISA. If the
antibody or antigen is not sticking to the plate, or unwanted
proteins are interfering with binding, the plate will have an
overall low specific signal. CB1 may foster adsorption and
reduce interference.
-
Decreases background noise. If excess proteins
are sticking to the plate, plate will have overall high
backgrounds (a high signal overall, but a low specific signal).
Universal Plate Coating Buffer may reduce interference.
|
How it Works: |
Our Coating Buffer is an optimal formulation of buffers and salts
which maximizes the adsorption of antibodies and antigens onto
polystyrene plates. During the adsorption process, the Coating
Buffer stabilizes coated proteins by maintaining their tertiary
three-dimensional structure. By stabilizing the adsorbed protein,
antigenic regions are preserved, allowing for greater binding
reactivity with the detection molecule, thereby enhancing the
specific signal. By generating a higher specific signal, a lower
concentration of coat protein may be needed when this buffer is
used, thereby saving valuable reagents.
|
Directions: |
Simply dilute Coating Buffer 1:5 (for example, add 100mL Coating
Buffer to 400mL diH20 for a final volume of 500mL) , add your
protein antigen or antibody, let the solution stir for 15 minutes,
and pipette onto the plate.
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ECB100 Universal Plate
Coating Buffer |
Cell Technology has specifically formulated a unique plate coating
buffer for use with antibodies and antigens. The pH and ionic
strength of this buffer have been specifically formulated to create
an optimal coating environment which maximizes the adsorption
efficiency of most antibodies and protein antigens onto polystyrene
plate surfaces. This buffer also stabilizes coated proteins,
preserving antigenic regions, and allowing for greater binding
reactivity with the detection molecule, enhancing the specific
signal. By generating a higher specific signal, a lower
concentration of coat protein may be needed when this buffer is
used, thereby saving valuable reagents.
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5x Universal Plate
Coating Buffer (ECB100)
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5x Bottle Size
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Price per Bottle
|
Part Number
|
Volume at 1x |
Plates at 50uL/well
(4.8mL/plate) |
Plates at 200uL/well
(9.6mL/plate) |
Plates at 300uL/well
(28.8mL/plate) |
100 mL
|
€40 |
644
|
500mL
|
100
|
25
|
16 |
500 mL
|
€95 |
645
|
2.5L
|
510 |
125 |
84 |
1 L |
€195.00 |
646
|
5L |
1020
|
250 |
170 |
10 L |
€1350.00 |
658 |
50L |
10,200
|
2,500 |
1,700 |
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