Home ] Up ] Call me back Contents Immunology Infectious agents Cellular Biology Molecular biology Instruments Chimical Product PCR ELISA Sites Komabiotech products ProductHome ] Up ] Phosphoprotein Enrich- Home | Leptin ELISA Kit | Human IGF-I ELISA Kit | Phosphoprotein Enrich- | ApoE Genotyping Kit | Hot Universal qMasterMix | One-Step RT-PCR MasterMix | Multiplex PCR MasterMix | Hot Taq PCR

Google

       Pricelist 2010 

Home
Up

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

EzWay Phos-Pro Enrichement Kit
Catalog Number K07920
Size 10T
Type Kit
Datasheet
Background
Protein phosphorylation is one of the most frequently occurred posttranslational modifications and plays a critical role in cellular regulatory events. Most cellular processes are in fact regulated by the reversible phosphorylation of proteins on serine, threonine and tyrosine residues. In fact, phosphorylation of proteins plays a key role in oncogenesis, cell signaling, apoptosis and immune disorders1.
Despite the importance and widespread occurrence of this modification, profiling of phosphoproteins in cells is still a challenge, due to the low copy of phosphorylated proteins in cell and the relative amount of phosphoproteins compared to unphosphorylated proteins.

Ladiolabeling by 32P labeling is frequently used conventional method for investigation of phosphoprotein profile in conjunction with 2-DE or 1-D gel electrophoresis and autoladiogram. Alternatively, western blot analysis probed by phosphoprotein-specific antibody is also used for this purpose.
Mass spectrometry has been shown to be a reliable and routine tool to identify proteins in a high throughput manner.
However, the identification of phosphorylation by mass spectrometry is not a trivial matter and to this day is not routine also due to the low copy of phosphorylated proteins in cells.

EzWay™ Phos-Pro Enrichment Kit provides advanced chance in detecting protein phosphorylation in gels with non-radiolabeling method(eg. Staining with fluorescence dye) and enables quantitative comparison between cells.

EzWay™ Phos-Pro Enrichment Kit is optimized for the protein solution in denatured condition, for example, the samples prepared for 2-DE and can be adapted to native proteins. Enriched phosphorylated proteins could be detected by staining commercially available staining method using fluorescent dye2 or by probing with antibodies, specific for phosphorylated proteins.

 
Features
  • Convenient fractionation of phosphoproteins from protein mixtures such as cell lysate or body fluids
  • Isolation and concentration of low copy phosphorylated proteins in cells
  • No use of IMAC column of phosphorylated antibodies
  • Downstream application for 2-DE or 1-D SDS-PAGE analysis
  • Enriched phosphorylated proteins detection by staining with fluorescent dye or antibody probing
  • Specificity 100%
  • Selectivity >87%
  • Sensitivity >93% / 50ng
  • Yield >93%
 
Kit Contents
  • Lysis Buffer
  • Dilution Buffer
  • Phos-Pro Solution A
  • Phos-Pro Solution B
  • Dissolving Solution
  • Delipidation Solution
 
How to Use
  1. Cell lysis and Protein extraction
  2. Precipitation of phosphoprotein complex
  3. Dedelipidation and recovering of phosphoprotein
 
Phosphoprotein Enrichment from Yeast cell Extract

CBB Staining

Phosphoprotein Staining
(ProQ Diamond)

 
  1. Sup of 8
  2. Sup of 9
  3. Sup of 10
  4. Sup of 11
  5. Sup of 12
  6. sup of 13
  7. Yeast lysate enriched (0.4mg/ml)
  8. Yeast lysate enriched (0.4mg/ml)
  9. Yeast lysate enriched (0.4mg/ml)
  10. Yeast lysate enriched (0.4mg/ml)
  11. Yeast lysate enriched (0.4mg/ml)
  12. Yeast lysate enriched (0.2mg/ml)
  13. Yeast lysate enriched (0.1mg/ml)
  14. Total cell Extract
 
 
Yeast total protein extract CBB Staining Enriched phosphorylated protein CBB staining Enriched phosphorylated protein ProQ Diamond Staining