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EzWay Phos-Pro Enrichement Kit
Protein phosphorylation is one of the most frequently occurred posttranslational modifications and plays a critical role in cellular regulatory events. Most cellular processes are in fact regulated by the reversible phosphorylation of proteins on serine, threonine and tyrosine residues. In fact, phosphorylation of proteins plays a key role in oncogenesis, cell signaling, apoptosis and immune disorders1.
Despite the importance and widespread occurrence of this modification, profiling of phosphoproteins in cells is still a challenge, due to the low copy of phosphorylated proteins in cell and the relative amount of phosphoproteins compared to unphosphorylated proteins.
Ladiolabeling by 32P labeling is frequently used
conventional method for investigation of phosphoprotein
profile in conjunction with 2-DE or 1-D gel
electrophoresis and autoladiogram. Alternatively,
western blot analysis probed by phosphoprotein-specific
antibody is also used for this purpose.
EzWay™ Phos-Pro Enrichment Kit provides advanced chance in detecting protein phosphorylation in gels with non-radiolabeling method(eg. Staining with fluorescence dye) and enables quantitative comparison between cells.
EzWay™ Phos-Pro Enrichment Kit is optimized for the protein solution in denatured condition, for example, the samples prepared for 2-DE and can be adapted to native proteins. Enriched phosphorylated proteins could be detected by staining commercially available staining method using fluorescent dye2 or by probing with antibodies, specific for phosphorylated proteins.
How to Use
Phosphoprotein Enrichment from Yeast cell Extract