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EzWay™ One-Step RT-PCR MasterMix
 
Catalog Number K0569600
Size 1ml
Type PCR Kit
Datasheet
 
Description
EzWay™ One-Step RT-PCR MasterMix is designed for sensitive and specific amplification in one-tube reaction from total RNA transcripts.
EzWay™ One-Step RT-PCR MasterMix is a premixed buffer solution containing MMLV Reverse Transcriptase and Hot Taq DNA Polymerase, dNTPs, tracking & loading dye and stabilizers. The solution contains the proper concentration of MgCl2 and dNTPs for the better specificity and sensitivity and supplied as 2X concentrated type for convenience.
Simply add primers and RNA template diluted in DEPC-treated water into equal volume of EzWay™ One-Step RT-PCR MasterMix. Pipetting steps are minimized, reducing the possibility of errors and contamination.
 
Features
  • Synthesis of cDNA and sequentially PCR amplification from target gene in one-tube.
  • Specialized buffer composition for highly sensitive and specific RNA amplificatio
  • Simple set-up and minimal optimization required
 
Application
Rapid detection of target gene transcript from total RNA or purified mRNA
 
Packing
Component Cat. No
2X EzWay™ One-Step RT-PCR MasterMix K0569600
4X Magic Buffer K0561031
 
RT-PCR of Beta-actin(275bp), c-Myc(215bp) and GAPD