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Recombinant human TRAIL-R1 (DR4/ TNFRSF10A)/Fc Chimera

  Product No. Form Quantity Stock Price Datasheet  
  RL-001-C050 Lyophilized 0.05 mg yes 290 EUR PDF datasheetHTML datasheet  
- In stock! Ready for immediate despatch - Manufacture on order

Source

Produced in HEK 293 cells. The extracellular domain of human TRAIL-R1 (aa 1 - 238) is fused to the Fc portion of human IgG1.

Description

DR4 is a transmembrane protein, also called TRAIL receptor 1 (TRAIL-R1). The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues.
Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

Database Links

Applications:

  • Loading control
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

References

  • Unpublished.
  •  
    For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse Monoclonal to CD261 / TRAIL-R1 DR-4-02 (IgG1)
     

    Example Data

    DR4 SDS
    Fig. 1. SDS-PAGE


    Fig.1. SDS-PAGE Electrophoresis of TRAIL-R1 (DR4) / Fc recombinant protein (12% separating gel; reducing conditions).
    Lane 1 to 3: TRAIL-R1 (DR4)/ Fc recombinant protein (2000 ng, 1000 ng, 500 ng per lane). Staining with Coomasie Blue.
     
    DR4
    Fig. 2. Western Blotting

    Fig.2. Western Blotting of TRAIL-R1 (DR4) / Fc recombinant protein. Reducing conditions.
    Lane 1 to 5: Immunostaining with TRAIL-R1 (DR4) specific antibody DR-4-02 (Cat. No. 10-403-C100; dilution 2 μg/ml ). Detection with Goat anti-mouse / HRP secondary antibody. Amount of TRAIL-R1 (DR4)/ Fc recombinant protein loaded on each lane: (1) 370 ng, (2) 150 ng, (3) 75 ng, (4) 37 ng, (5) 15 ng.

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