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Native human Prostate-specific antigen / PSA

  Product No. Form Quantity Stock Price Datasheet  
  NL-008-C100 Lyophilized 0.1 mg yes choose region PDF datasheetHTML datasheet  
- In stock! Ready for immediate despatch - Manufacture on order

 

Source

Prostate-specific antigen (PSA) was purified from human seminal plasma by ion-exchange chromatography and gel filtration. The purified antigen was dialyzed against d.H2O and lyophilized.

Description

Prostate-specific antigen (PSA) is a glycoprotein of approx. 30 kDa found mainly in prostatic tissue and seminal fluid; it is produced by normal prostate cells. The molecule consists of approximately 92% protein and 8% carbohydrate. This enzyme participates in the dissolution of the seminal fluid coagulum and plays an important role in fertility. The highest amounts of PSA are found in the seminal fluid; some PSA escapes the prostate and can be found in the serum. This serum component has been used to track the response to therapy in men with prostate cancer.

Database Links


 

Applications:

  • Positive control
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Zhang WM, Leinonen J, Kalkkinen N, Dowell B, Stenman UH: Purification and characterization of different molecular forms of prostate-specific antigen in human seminal fluid. Clin Chem. 1995 Nov;41(11):1567-73. [Abstract]
  •  
    For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse Monoclonal to Prostate-specific antigen (PSA) A67-B/E3 (IgG1)
  • Mouse Monoclonal to Prostate-specific antigen (PSA) A67-B/E3 (IgG1)
     

    Example Data

     


     
     
    PSA antigen
    Fig. 1. Western Blotting


     

     

    Fig.1. Western Blotting analysis of native human Prostate-specific antigen (cat. no. NL-008-C100). Reducing conditions.
    Lane 1-3: immunostaining of PSA antigen (1 mg/ml) with anti-human Prostate-specific antigen antibody (A67-B/E3; EXBIO cat. no. 11-289-C100; dilution 1 μg/ml (1), 0.5 μg/ml (2), 0.2 μg/ml (3)). Detection by Goat anti-mouse / HRP secondary antibody.

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