The Exalpha Biologicals, Inc. Phospho Extracellular
signal-Regulated Kinase 1/2 (ERK 1/2) ELISA is a
non-isotopic immunoassay for the in vitro quantitation
of human phospho ERK 1/2 (T202/Y204-T185/Y187) protein
in cell lysates. Also called p42/p44 or Mitogen
Activated Protein Kinase 1/2 (MAPK1/MAPK2).
Features - Colorimetric assay
- HTS compatible format
- High sensitivity
- 4 deg C storage with long shelf life
- 5 hour protocol
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CHO cells transfected with the human S1P4 receptor for S1P
(Edg6) were serum stared for 4 hours and challenged with S1P
or LPA. PMA was used as a positive control for Phospho ERK
1/2 production. PHOSPHO ERK 1/2 was detemined to be an
excellent surrogate marker for stimulation with S1P compared
with traditional methods for GPCR signaling such as
intracellular Calcium flux.
Protocol Summary: Not to be used in
place of detailed protocol. See kit manual for complete protocol.