Home ] Up ] Call me back Contents Immunology Infectious agents Cellular Biology Molecular biology Instruments Chimical Product PCR ELISA Sites Komabiotech products ProductHome ] Up ] EzWay DAB Western Blot Kit Home | EzWay DAB Western Blot Kit | EzWay IP (Immunoprecipitation) Kit | EzWay Annexin V-FITC Apoptosis Detection Kit | EzWay Antibody Biotin Labeling Kit

Google

       Pricelist 2010 

Home
Up

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

EzWay DAB Western Blot Kit
Catalog Number K03350
Size 10A
Type Kit
Datasheet
 
Specification
  • Specific and accurate colorimetric protein detection using DAB
  • Optimized all reagents supplied for quick and convenient western blot assay
  • Affinity purified secondary antibodies included for versatility (rabbit & mouse)
  • High sensitivity and clear background
  • Easy to use
 
Protocol
Transfer >> Rinsing >> Blocking >> Washing >> Reacting with primary antibody >> Washing >> Reacting with secondary antibody >> Washing >> Color reaction
 
Kit Contents
Items Quantity
25X Transfer Buffer (Tris-Glycine)** 100ml x 2
6X Blocking Solution (3% Casein/PBS) 120ml x 2
PBS Powder Pouch for 1L x 5
Tween-20 (50%) 1ml (50%) x 5
30X DAB Solution 15ml
Substrate solution (30% H2O2) 1ml
Secondary antibody (goat anti-mouse IgG-HRP)*/** 0.1ml
Secondary antibody (goat anti-rabbit IgG-HRP)*/** 0.1ml
* The dilution rate of secondary antibodies for WB is 1:1,000-1:10,000. They are also applicable to use for ELISA (1:10,000-1:100,000) as well as IHC (1:200-1:500).
** For separate purchasing, this small volume is not available.
 
Western Blot
Immunoblot Analysis Marker (lane 1), 10 ug (lane 2), 1 ug (lane 3), 0.1 ug (lane 4), 0.01 ug (lane 5) of BSA samples were resolved by lectrophoresis, blotted to nitrocellulose, and probed with Anti-BSA (1:4,000). Proteins were visualized with at anti-rabbit secondary antibody conjugated to HRP (1:5,000) and DAB substrate. Arrow indicates BSA (69 kDa).
 
 
Dot Blot
10 ug (lane 1), 1 ug (lane 2), 0.1 ug (lane 3), 0.01 ug (lane 4), 0.001 ug (lane 5) of BSA samples were dotted to nitrocellulose, and probed with Anti-BSA (1:4,000). Proteins were visualized with goat anti-rabbit secondary antibody conjugated to HRP (1:5,000) and DAB substrate.