27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
EzWay Transfection Reagent
EzWay™ Transfection Reagent, consisting of a unique cationic lipid has appropriate DNA-binding affinity for reliable gene transfection that is applicable to both preclinical and nonclinical studies. Preparing the liposome-DNA complex (lipoplex) is very easy and rapid.
Also, EzWay™ Transfection Reagent shows relatively stronger DNA-binding affinity and better transfection efficiency than other liposomal reagents.
Sufficient reagent for 250 transfection in a 24-well plate is provided.
List of cell lines successfully transfected with EzWayTM Transfection Reagent
Efficient delivery of DNA to a wide variety of cell lines
Using the EzWay™ Transfection Reagent or competitor reagent L, cells were transfected with the pDNA, which expresses luciferase (A) and GFP (B) respectively. One microgram of pDNA complexed to EzWay ™(1:4.9 wt ratios of pDNA and lipid) was added to a various cell lines, The cells were transfected for 4 hours and incubated for additional 24 hours. The luciferase activity (A) of the cell lysate was measured with a luminometer and a luciferase assay kit and the GFP expression (B) was determined by fluorescence microscopy.
Knockdown efficacy of EzWay™ Transfection Reagent
siRNA targeting luciferase was transfected by EzWay™ Transfection Reagent and competitor reagent L into Hep-3B cells. For B16BL6 cells, siRNA targeting luciferase was co-transfected with luciferase gene by two products, respectively. Luciferase-silencing efficiency was calculated relative to untreated cells. (A) shows the luciferase expression, while (B) shows the reduction in luciferase activity. EzWay™ Transfection Reagent delivered significant gene silencing from 10 nM of renilla luciferase siRNA.
Inhibition of GFP expression in cells transfected with GFP targeting siRNA using EzWay™ Transfection Reagent
MDA-MB-231 cells constitutively expressing the GFP gene were transfected with 10 nM final siRNA concentration of GFP-targeting siRNA.