Google

       Pricelist 2010 

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

EzWay Transfection Reagent
Catalog Number K21000
Size 0.5ml
Type Buffer & Reagent
Datasheet
Specification
EzWay™ Transfection Reagent, consisting of a unique cationic lipid has appropriate DNA-binding affinity for reliable gene transfection that is applicable to both preclinical and nonclinical studies. Preparing the liposome-DNA complex (lipoplex) is very easy and rapid.
Also, EzWay™ Transfection Reagent shows relatively stronger DNA-binding affinity and better transfection efficiency than other liposomal reagents.
Sufficient reagent for 250 transfection in a 24-well plate is provided.
  • Cationic lipid liposome-based reagent
  • Low cellular toxicity
  • Efficient siRNA and plasmid DNA delivery
  • Transfection of a wide variety of cell types
  • High transfection efficiency of stable and transient transfections
  • Effective in both serum-containing medium and serum-free medium
  • Ready-to-use reagent
  • Simple protocol
  • Universal use for both in vivo and in vitro
 
Protocol
 
Applications
List of cell lines successfully transfected with EzWayTM Transfection Reagent
Cell Line Cell Type
HeLa Human cervix adenocarcinoma
293A Human kidney cancer
B16BL6 Mouse skin melanoma
B16F10 Mouse skin melanoma
MCF-7 Human breast adenocarcinoma
HepG2 Human liver/hepatocellular carcinoma
Hep-3B Human hepatocellular carcinoma
Chang Human normal liver cell
BT-20 Human breast carcinoma
TC-1 Mouse lung tumor
CT-26 Mouse colon carcinoma
EMT-6 Mouse breast cancer
SK-OV-3 Human ovarian adenocarcinoma
A549 Human lung epithelial carcinoma
MDA-MB-231 Human breast adenocarcinoma
NCI Human lung cancer (carcinoma)
SK-Br-3 Human breast carcinoma
HT-29 Human colorectal adenocarcinoma
Jurkat Human T-cell leukemia
 
Efficient delivery of DNA to a wide variety of cell lines
(A)
(B)
Using the EzWay™ Transfection Reagent or competitor reagent L, cells were transfected with the pDNA, which expresses luciferase (A) and GFP (B) respectively. One microgram of pDNA complexed to EzWay ™(1:4.9 wt ratios of pDNA and lipid) was added to a various cell lines, The cells were transfected for 4 hours and incubated for additional 24 hours. The luciferase activity (A) of the cell lysate was measured with a luminometer and a luciferase assay kit and the GFP expression (B) was determined by fluorescence microscopy.
 
Knockdown efficacy of EzWay™ Transfection Reagent
siRNA targeting luciferase was transfected by EzWay™ Transfection Reagent and competitor reagent L into Hep-3B cells. For B16BL6 cells, siRNA targeting luciferase was co-transfected with luciferase gene by two products, respectively. Luciferase-silencing efficiency was calculated relative to untreated cells. (A) shows the luciferase expression, while (B) shows the reduction in luciferase activity. EzWay™ Transfection Reagent delivered significant gene silencing from 10 nM of renilla luciferase siRNA.
 
Inhibition of GFP expression in cells transfected with GFP targeting siRNA using EzWay™ Transfection Reagent
MDA-MB-231 cells constitutively expressing the GFP gene were transfected with 10 nM final siRNA concentration of GFP-targeting siRNA.