Home ] Up ] Call me back Contents Immunology Infectious agents Cellular Biology Molecular biology Instruments Chimical Product PCR ELISA Sites Komabiotech products ProductHome ] Up ] NADP/NADPH Detection Home | NAD/NADH Detection Kit | Fluorescent Thiol Detection Kit | NADP/NADPH Detection

Google

       Pricelist 2010 

Home
Up

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                   

Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o.

Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

OUR SUPPLIERS

KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE
Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic

 

 

Fluoro NADP
Fluorescent NADP/NADPH Detection Kit - Detection of NADP/NADPH in tissue extracts /cell samples
 
Key Benefits:
  • Detection of NADP/NADPH content in cells or tissue extracts
  • Detection of NADP/NADPH levels in antioxidation and oxidative stress
  • Detection of NADP/NADPH levels in cell death, energy metabolism, and mitochondria function
  • Species Independent - NADP/NADPH detection in Bacterial, fungal and plant cells
  • Highly Sensitive – Detects up to 4nM NADP and NADPH
  • Highly Specific - No Cross reactivity with NAD/NADH
  • Easy to Use – 96 well Fluorescent Plate reader readout
     
Introduction

Nicotinamide adenine dinucleotide phosphate (NADP+) is used in anabolic reactions, such as lipid and nucleic acid synthesis, which require NADPH as a reducing agent. NADPH is the reduced form of NADP+, and NADP+ is the oxidized form of NADPH. In cells, NADPH plays the role of a carrier of reducing power and is primarily involved in maintaining optimal redox metabolism. A simplified assay for the measurement of NAD and NADP is critical to understanding the roles of these pyridine nucleotides in normal and abnormal cells.

NADPH is produced in the oxidative phase of the pentose phosphate pathway in cells, a multifunctional pathway whose primary purpose is to generate reducing power in the form of NADPH. NADPH is a cofactor for enzymes that synthesize energy-rich molecules and provide the reducing equivalents for the oxidation-reduction involved in protecting the cell from the toxicity of reactive oxygen species (ROS) and NADPH oxidase-dependent ROS generation. Both NAD and NADP have been shown to influence hemoglobin affinity for oxygen in erythrocytes. In plant cells, NADPH is used as the reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis (1-2).

Cell Technology’s Fluoro NADP/NADPH provides a highly reliable, sensitive fluorometric assay for the quantification of NADP, NADPH and their ratio in biological samples.

Assay Principle
The Fluoro NADP/NADPH detection kit utilizes a non-fluorescent detection reagent, which is oxidized in the presence NADPH to produce its fluorescent analog and NADP. NADP is further converted to NADPH via an enzyme-coupled reaction. The enzyme reaction specifically reacts with NADP/NADPH and not with NAD/NADH.

Reaction:

1. NADPH + non-fluorescent detection reagent + electron coupler → fluorescent analog + NADP

2. NADP + enzyme coupled reaction →  NADPH (then proceeds to reaction 1).

   Excitation: 530-570nm and Emission at 590-600nm
 
References
1. Comprehensive Invited Review WEIHAI YING. NAD /NADH and NADP /NADPH in Cellular Functions and Cell Death: Regulation and Biological Consequences. ANTIOXIDANTS &REDOX SIGNALING Volume 10,Number 2,2008.

2. Bedard K and Krause KH. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology.Physiol Rev 87:245 –313,2007.

3. Lowry, Oliver H., Passonneau, Janet V. and Rock, Martha K. The Stability of Pyridine Nucleotides. The Journal of Biological Chemistry, 236, #10, 1961.
Kit contents (for 100 assays)
1. Enzyme Mix 170μL                          Part  6022 .......................... 4-8C 

2. NADP/NADPH Lysis Solution 12.5mL   Part 3050 ............................4-8C

3. NADP Extraction Buffer 22 mL           Part 3046 (Green label) ..........4-8C

4. NADPH Extraction Buffer 22 mL         Part 3047 (Magenta label)........4-8C

5. Reaction Buffer 11 mL.                    Part 3049 ............................4-8C

6. Standard curve diluent 15 ml.            Part  3052............................4-8C

7. NADPH Detection reagent 110 μL       Part  4019...........................-20C 

8. 3X Substrate Mix 6mL                     Part  3048........................... -20C 

9. NADPH Standard- 3 vials dried          Part 7014.............................-20C
 
The following kits are available:
Catalog
 
Size
 
 Price (US$)
NADPH 100-2 100 Tests $495

Comparison of NADPH & NADH Std Curves

 Comparison of NADP & NADPH Std Curves

 

NADP and NADPH levels were assayed using the Fluoro NADP/NADPH kit. Yield from 6 X105 Jurkat cells (n=3).