Can monitor multiple time points to follow
One-step, no wash assay.
Adaptable for High Throughput format
Applications - Fluorescence (Plate
Reader) or Absorbance
Myeloperoxidase (MPO) is a highly cationic
glycosolated hemoprotein that has a molecular weight
of 144kD. The hemoprotein consists of two dimers
linked via a disulfide bridge. Each dimmer is
composed of a heavy (53kD) and light (15kD) subunit.
Each heavy chain contains an independently acting
protoporphyrin group containing a central iron
(1-5). MPO is present in the azurophilic
granules of polymorphonuclear leukocytes (PMNs) and
is unique to neutrophils and monocytes. However,
monocytes contain only one third of the MPO found in
PMN’s. MPO utilizes H202 produced by the neutrophils
to oxidize a varity of aromatic compounds to give
substrate radicals for bactericidal activity (4
review). This enzyme is unique however in that
it can oxidize chloride ions to produce a strong
nonradical oxidant,HOCl. HOCl is the most powerfull
bactericidal produced by neutrophils (4 review).
Excessive production of these radicals can cause
oxidative stress leading to oxidative tissue injury.
Detection of MPO activity in neutrophils and
Detection of PMN infiltration in tissue samples
(inflammation and innate host defense mechanisms).
Acute and chronic inflammatory disorders due to
oxidative tissue damage.
MPO activity in acute and chronic manifestations
of cardiovascular disease.
following kits are available:
Figure 1. MPO standard curve was
serially diluted in 1X Reaction Buffer.
Reaction cocktail (RC) was prepared as
described (without EPO inhibitor). Next
50mL of MPO standard and 50mL of RC was
added to individual well of a 96 well
black plates. The plate was incubated at
room and temperature in the dark. Data
collected Ex:530nm Em:590nm