27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
5-15 units/ul. Assayed on unmethylated λ DNA.
Moraxella bovis ATCC10900
[6mM Tris-HCl (pH7.5 @ 37°C), 6mM MgCl2, 50mM NaCl, 1mM DTT]
Incubate at 37°C
No. of Cleavage Sites
Blocked by overlapping dam methylation. Single base extension produced by Mbo II digestion is difficult to ligate.