- Staining Occurs During the Run
- Ideal Prep for Mass Spec
- 10ng Sensitivity in 30 Minutes!
Insite System is an in-gel
fluorescent stain for proteins,
providing excellent sensitivity
(10ng) more quickly than any other
detection method. The Insite stain
runs with the proteins during
electrophoresis. No staining step is
required after electrophoresis. To
use the Insite System, researchers
simply mix Insite Buffer Concentrate
with Insite Dye Concentrate and QS
with deionized water to formulate
their cathode tank buffer for SDS-PAGE.
The Insite dye runs with the
proteins during electrophoresis.
After a short destain in water
following electrophoresis, the bands
are visualized under UV
Proteins stained with the Insite
System may be easily and
reproducibly recovered at high yield
from SDS-PAGE gels for analysis by
The image on the left above shows
the mass spec of Carbonic Anhydrase.
Above right, the image shows the
mass spec of Carbonic Anhydrase that
has been electrophoresed by SDS-PAGE,
visualized by the Insite System, and
Purified from the Gel Slice by Crush
and Soak Technique.
The Insite System contains
sufficient material to stain 60
Catalog Number: EC-759