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Hydroxyl / Peroxynitrite Detection Kit
Fluorescent Hydroxyl (•OH) / Peroxynitrite (ONOO -) Detection Kit
Key Benefits:
  • Quenched Cell permeable dye
  • Can be used with Cell Lysates, Tissue Homogenates
  • One Step, No wash Homogenous assay.
  • Adaptable to High throughput assay platforms
  • Can monitor multiple time points to follow real time kinetics
  • Non-destructive cell based assay allows monitoring of additional parameters
  • Applications - Fluorescence plate reader / Flow cytometer / Fluorescence Microscope
Introduction:
A new novel probe, Hydroxyphenyl fluorescein (HPF), developed by Tetsuo Nagano et. al. (1), is a highly selective probe for the detection of highly Reactive Oxygen Species (hROS). It is a cell permeable highly sensitive fluorescent probe for hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (021), superoxide (02-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1.
Assay Principle:
Reactivity:
Reactivity Profile of HPF:
ROS (RFU)
 		 HPF (RFU)
Ex:499 Em:515
 		 DCFH-DA (RFU)
Ex:500 Em:520
 
Hydroxyl Radical: •OH
 		 730 7400
Peroxynitrite: ONOO- 120
 		 6600
Hypochlorite: -OCl 6 86
Oxygen Radical: 1O2 5 26
Superoxide: O2-• 8 67
Hydrogen Peroxide : H202 2 190
Nitric Oxide: NO 6 150
Alkylperoxyl Radical: ROO• 17 710
Autoxidation 1 2000
The following kits are available:
Catalog #
 		 Contents Size* Price
FLHPF 100-2
 		 HPF 1 vial 150 Tests * €195
Dilution of HPF
 		 Volume of test Numer of tests
1:500 (10mM final)
 		 0.2 mL 147
1: 1000 (5mM final) 0.2mL 294