A new novel probe, Hydroxyphenyl fluorescein (HPF),
developed by Tetsuo Nagano et. al. (1), is a highly
selective probe for the detection of highly Reactive Oxygen
Species (hROS). It is a cell permeable highly sensitive
fluorescent probe for hydroxyl radical (OH•),
and peroxynitrite (ONOO-)
detection. It has little reactivity towards other hROS such
as: hypochlorite (-OCl),
singlet oxygen (021),
superoxide (02-•),
hydrogen peroxide (H202), nitric oxide
(NO•),
and alkyl peroxide (RO2•)
(see table below)1.
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