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Hot Taq DNA Polymerase Kit
Catalog Number K0567100
Size 250U
Type PCR Kit
Datasheet
 
Description
Hot Taq DNA Polymerase, a chemically modified form of Taq DNA Polymerase, is supplied in an inactive state that has no polymerase activity at ambient temperatures. This prevents extension of nonspecifically annealed primers and primer–dimers formed at low temperatures during PCR setup and the initial PCR cycle. This makes Hot Taq DNA Polymerase ideal for quantitative real-time PCR. Hot Taq DNA Polymerase is activated by 15-minute incubation at 95°C which can be incorporated into any existing thermal-cycler program. Every lot of Hot Taq DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified.
 
Features
  • High specificity
  • Reduced non-specific amplification
  • High reproducibility
  • Extension rate: 2-4 kb/min at 72°C
  • Half-life: 10 min at 97°C, 60 min at 94°C
  • Amplification efficiency: ≥105 fold
  • 5'→3' exonuclease activity
  • Extra A addition
  • Contaminating nucleases: No
  • Contaminating RNases: No
  • Contaminating proteases: No
  • Self-priming activity: No
 
Applications
  • Highly Specific PCR
  • Low Copy Number Target PCR.(e.g. Viral Detection in Blood)
  • RT-PCR of rare transcript
  • Real-time PCR
  • Differential Display
  • Multiplex PCR, PCR-based DNA fingerprinting (VNTR, STR, and RAPD)etc
  • Degenerate PCR
 
Packing
Component Cat. No
Hot Taq DNA Polymerase K0567100
10X Hot Taq PCR Buffer K0567111
dNTP Mix (2.5mM each) K1756011
10X Loading Buffer K0561051
4X Magic Buffer K0561031