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EzWay Protein-Silver Staining Kit
Catalog Number K14040
Size 20A
Type Buffer & Reagent
Datasheet Inquire
 
Specification
  • A rapid and easy method for staining proteins in polyacryamide gels
  • 30 times more sensitive than Coomassie staining method
  • No background and free from dark spots
  • Complete destaining of gels within 15 minutes
  • Unique buffers and protocol for preparation of protein sample for MALDI-TOF-MS analysis
    (no glutaraldehyde included)
  • No. of applications: 20 mini-gels
  • Storage : 6 months at RT
  • Sensitivity: Detects 0.1 ng of BSA
 
Kit Contents
  • Sensitizer
  • Stainer A, B
  • Developer
  • Developer Enhancer
  • Destainer A, B
 
Protocol
  1. After electrophoresis, rinse the gel with DW.
  2. Fix the gel in the fixative solution and then wash the gel.
  3. Incubate the gel in the Sensitizer.
  4. Make a staining solution by mixing Stainer A & B, and incubate the gel in this solution for 30 minutes. Wash the gel.
  5. Make a developing solution by mixing Developer & Developer Enhancer, and incubate the gel until bands start to appear and the desired band intensity is reached.
  6. To stop the reaction, pour 100% acectic acid to the gel and agitate.
  7. If you need to destain the gel for reducing background, use the mixed solution of Destainer A & B.