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EzWay Neo Taq PCR MasterMix (2x)
Catalog Number K0565511 K0565515
Size 1ml 5ml
Type PCR Kit PCR Kit
Datasheet
 
Description
The EzWay™ Neo Taq PCR MasterMix is a mastermix containing Neo Taq DNA Polymerase, Neo Taq Buffer, dNTPs and MgCl2. The EzWay™ Neo Taq PCR MasterMix is a blend of two thermal DNA polymerases, therefore, the fidelity and amplification length of the resultant DNA fragment is improved, while retaining the DNA polymerization power of the Taq DNA Polymerase. Simply add 1 volume of primers and template DNA diluted in the distilled water into equal volume of 2x EzWay™ Neo Taq PCR MasterMix. Pipetting steps are minimized, reducing the possibility of errors and contamination.
The combination of long range amplification and easy handling makes the 2x EzWay™ Neo Taq PCR MasterMix ideal for use with rare and long size DNA synthesis from cDNA libraries, 5'-RACE, 3'-RACE, and amplification of >3kb insert for cloning.
 
Features
  • Premix solution of Neo Taq DNA Polymerase, Neo Taq PCR buffer, dNTPs, MgCl2 and additives in a 1.5ml tube
  • A blend of Taq DNA Polymerase and a thermostable DNA Polymerase with proofreading activity
  • Amplification range:
    • Lambda DNA: up to 20Kb
    • Human genomic DNA: up to 10Kb
  • Efficient amplification of low copy number targets and long fragment
  • Customer's optional aliquot
  • A time saving preparation
  • Reduced risk of handling errors and contamination
  • 5'→3' exonuclease activity
  • 3'→5' exonuclease activity
  • Extra A addition (terminal transferase) activity
  • Higher fidelity than standard Taq DNA polymerase
  • High enzyme activity
  • High reproducibility
  • Easy handling and shipping due to temperature stability
 
Application
  • Amplification of low copy number targets
  • Amplification of long range genomic DNA and cDNA targets
  • Moderate fidelity and TA/blunt end cloning
  • RT-PCR with a longer or rare transcript (>3Kb)
 
Storage
1 year at -20°C
(The product is able to be shipped on blue ice and should be stored immediately at -20°C.)
 
Packing
Component Cat.No Packing
2X EzWay™ Neo Taq PCR Master Mix K0565511 1.0ml
4X Magic Buffer K0561031 1.0ml