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EzWay DNA Methylation Detection Express Kit
Catalog Number K03750
Size 20T
Type Kit
Datasheet
 
Specification
The detection of methylation in CpG island of genomic DNA is one of the most important fields in epigenetic studies. Koma introduces a new EzWay™ DNA methylation detection kit that converts unmethylated cytosine into uracil and leaves methylated cytosine as it is by Na-bisulfite.
EzWay™ DNA methylation detection kit is ready to use kit including DNA extraction kit, DNA binding and elution buffer, Na-bisulfite. The advantages of this kit lies in very high recovery rate that comes from the proprietary binding and elution buffer for Na-bisulfite treated DNA.
This advanced EzWay™ DNA methylation Detection Express Kit converts unmethylated cytosine into uracil and leaves methylated cytosine as it is by Na-bisulfite in only 4 hours.
  • Bisulfite modification & spin column method
  • Complete conversion of unmethylated cytosine into uracil
  • A fast C/U conversion step using heat denaturation
  • Just 3-4 hours for whole procedures
  • High recovery rate of modified DNA sample
  • More simple DNA purification & elution procedure
  • Sufficient volume of DNA Binding Buffer is supplied
  • DNA Purification Kit is included
  • A control primer is supplied for confirmation of DNA modification
 
Applications
  • MSP (Methylation specific PCR)
  • Methylation detection using DNA chip
  • COBRA (combined bisulfite restriction assay)
  • Methylate and Bisulfite sequencing etc.
 
Kit Contents
  • C/U Conversion Reagent I
  • C/U Conversion Reagent II
  • D Reagent
  • DNA binding Buffer
  • DNA desalting Buffer
  • Desulphonation Buffer
  • Elution Buffer
  • DNA Prep Columns
  • Collection Tubes
  • Control primer
 
Protocol
 
Principle