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EzWay DAB Substrate Kit
Catalog Number K0335040
Size 10A
Type Buffer & Reagent
Datasheet
 
Specification
  • Diaminobenzidine (DAB) is the commonly used substrate for detection of peroxidase.
  • 2 component system which develops a brown precipitate when reacted with horseradish peroxidase (HRP) in western blotting.
 
Protocol
  1. Add 1 volume of 30X DAB Solution to 29 volumes of PBS and add 30 ul Substrate Solution (30% H2O2) per 30 ml 1X DAB solution prior to use.
  2. Incubate the membrane in the color reaction mixture for several minutes at room temperature or at 37°C.
  3. After sufficient color development, wash the membrane with DW in order to stop the color reaction.
 
Kit Contents
Items Quantity
Quantity 15 ml
Substrate solution (30% H2O2) 1ml